Journal: Neuroscience research

Article Title: Long-lasting expression of FosB/ΔFosB immunoreactivity following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus.

doi: 10.1016/j.neures.2025.104911

Figure Lengend Snippet: Figure 1. Time courses of c-Fos (Figure 1A) and FosB/∆FosB expression (Figure 1B)

Article Snippet: The free-floating sections were washed with 0.01 M PBS and incubated with goat polyclonal anti-FosB antibody (Santa Cruz, Cat# sc-48-G, RRID AB_631516) at 1:1000 and either of the following antibodies: rabbit polyclonal anti-CRF antibody at 1:5000 (#PBL rC70, generously donated by Dr. W. Vale, Salk Institute), rabbit polyclonal anti-AVP antibody at 1:5000 (#R, Dr. T. Mouri, Tohoku University) (Mouri et al., 1993), or rabbit polyclonal anti-OXT antibody at 1:5000 (Millipore, Cat# AB911, RRID AB_2157629).

Techniques: Expressing

Journal: Neuroscience research

Article Title: Long-lasting expression of FosB/ΔFosB immunoreactivity following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus.

doi: 10.1016/j.neures.2025.104911

Figure Lengend Snippet: Figure 2. Time courses of c-Fos and FosB/∆FosB expression in the PVH following

Article Snippet: The free-floating sections were washed with 0.01 M PBS and incubated with goat polyclonal anti-FosB antibody (Santa Cruz, Cat# sc-48-G, RRID AB_631516) at 1:1000 and either of the following antibodies: rabbit polyclonal anti-CRF antibody at 1:5000 (#PBL rC70, generously donated by Dr. W. Vale, Salk Institute), rabbit polyclonal anti-AVP antibody at 1:5000 (#R, Dr. T. Mouri, Tohoku University) (Mouri et al., 1993), or rabbit polyclonal anti-OXT antibody at 1:5000 (Millipore, Cat# AB911, RRID AB_2157629).

Techniques: Expressing

Journal: Neuroscience research

Article Title: Long-lasting expression of FosB/ΔFosB immunoreactivity following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus.

doi: 10.1016/j.neures.2025.104911

Figure Lengend Snippet: Figure 6. The time courses of c-fos-, fosB-, and ΔfosB mRNA levels following surgical

Article Snippet: The free-floating sections were washed with 0.01 M PBS and incubated with goat polyclonal anti-FosB antibody (Santa Cruz, Cat# sc-48-G, RRID AB_631516) at 1:1000 and either of the following antibodies: rabbit polyclonal anti-CRF antibody at 1:5000 (#PBL rC70, generously donated by Dr. W. Vale, Salk Institute), rabbit polyclonal anti-AVP antibody at 1:5000 (#R, Dr. T. Mouri, Tohoku University) (Mouri et al., 1993), or rabbit polyclonal anti-OXT antibody at 1:5000 (Millipore, Cat# AB911, RRID AB_2157629).

Techniques:

Journal: Neuroscience research

Article Title: Long-lasting expression of FosB/ΔFosB immunoreactivity following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus.

doi: 10.1016/j.neures.2025.104911

Figure Lengend Snippet: Figure 7. The time courses of the number of c-Fos-ir and FosB/∆FosB-ir neurons, and

Article Snippet: The free-floating sections were washed with 0.01 M PBS and incubated with goat polyclonal anti-FosB antibody (Santa Cruz, Cat# sc-48-G, RRID AB_631516) at 1:1000 and either of the following antibodies: rabbit polyclonal anti-CRF antibody at 1:5000 (#PBL rC70, generously donated by Dr. W. Vale, Salk Institute), rabbit polyclonal anti-AVP antibody at 1:5000 (#R, Dr. T. Mouri, Tohoku University) (Mouri et al., 1993), or rabbit polyclonal anti-OXT antibody at 1:5000 (Millipore, Cat# AB911, RRID AB_2157629).

Techniques:

Journal: Neuroscience research

Article Title: Long-lasting expression of FosB/ΔFosB immunoreactivity following acute stress in the paraventricular and supraoptic nuclei of the rat hypothalamus.

doi: 10.1016/j.neures.2025.104911

Figure Lengend Snippet: Figure 8. Co-localization of FosB/∆FosB immunoreactivity with neuroendocrine

Article Snippet: The free-floating sections were washed with 0.01 M PBS and incubated with goat polyclonal anti-FosB antibody (Santa Cruz, Cat# sc-48-G, RRID AB_631516) at 1:1000 and either of the following antibodies: rabbit polyclonal anti-CRF antibody at 1:5000 (#PBL rC70, generously donated by Dr. W. Vale, Salk Institute), rabbit polyclonal anti-AVP antibody at 1:5000 (#R, Dr. T. Mouri, Tohoku University) (Mouri et al., 1993), or rabbit polyclonal anti-OXT antibody at 1:5000 (Millipore, Cat# AB911, RRID AB_2157629).

Techniques:

Journal: bioRxiv

Article Title: Repetitive Neuronal Activation Regulates Cellular Maturation State via Nuclear Reprogramming

doi: 10.1101/2025.05.02.651848

Figure Lengend Snippet: a , Top, ΔFosB immunostaining in the DG. Scale bar, 500 μm. Bottom, time course of ΔFosB expression. Day 0 denotes the first stimulation day. Values were normalized to the mean of the No Stim group. The black dashed line indicates the theoretical decay of ΔFosB (half-life = 208 h); blue and red curves represent exponential fits for the Stim×3 and Stim×10 groups, respectively. n = 9–11 slices per group. One-sample t- tests were performed between observed values and the theoretical decay curve; *** P < 0.001. b, Immunostaining of ΔFosB and calbindin in the DG (same data as in panel a). Only No Stim and Stim×10+40 days are shown. Scale bar, 20 μm. Right, scatter plot showing the correlation between ΔFosB and calbindin immunoreactivity. c , ΔFosB immunostaining in the DG of Scram-KO and CyclinB-KO mice (same mice as in ). Bottom panels show enlarged views of the white boxes. Grayscale indicates Hoechst staining. Scale bar, 20 μm. Beeswarm showing ΔFosB immunoreactivity (n = 129–165 cells per group). Black bars indicate the median. One-way ANOVA: F (5, 859) = 270.94, P < 2.0 × 10[¹[; post hoc comparisons with Tukey’s test. d, Schematic of Cyclin B/Cdk1 activation (modified from Deibler et al. 2010 ). Cdk1 is activated as cyclin B accumulates. This activation is inhibited by Wee1 and promoted by Cdc25c. e, Top, ΔFosB immunostaining in the DG. Middle, enlarged view of the boxed region. Grayscale indicates Hoechst staining. Bottom, calbindin immunostaining. Scale bar, 20 μm. f, Beeswarm plots showing ΔFosB, calbindin, and chromocenter ratio. n = 114–156 cells per group. Black bars indicate the median. One-way ANOVA: ΔFosB: F (4, 649) = 405.45 , P < 2.0 × 10[¹[; Calbindin: F (4, 655) = 117.65, P < 2.0 × 10[¹[; Chromocenter ratio: F (4, 655) = 14.63, P < 2.0 × 10[¹[. Multiple comparisons were corrected using Tukey’s test.

Article Snippet: The sections were stained with the following antibodies: Calbindin (1:1000, rabbit, polyclonal, Synaptic Systems Cat# 214 002, RRID:AB_2068199), Cyclin B (1:500, mouse, monoclonal, Thermo Fisher Scientific Cat# MA1-155, RRID:AB_2536863), phospho-Histone H3 (1:500, rabbit, polyclonal, Millipore Cat# 06-570, RRID:AB_310177), HA-tag (1:500, rabbit, polyclonal, Cell Signaling Technology Cat# 3724, RRID:AB_1549585), ΔFosB (1:1000, rabbit, monoclonal, Cell Signaling Technology Cat# 14695, RRID:AB_2798577), Calbindin (1:1000, rabbit, polyclonal, Synaptic Systems Cat# 214002, RRID:AB_2068199), NeuN (1:500, mouse, monoclonal, Millipore, Cat# MAB377X, RRID:AB_2149209), GFAP (1:500, rabbit, polyclonal, Sigma-Aldrich, Cat# G9269, RRID:AB_477035), Iba1 (1:500, rabbit, polyclonal, FUJIFILM, Cat# 019-19741, RRID:AB_839504), JunD (1:500, rabbit, polyclonal, Abcam, Cat# ab28837, RRID:AB_2130167), and tri-methyl-Histone H3 (Lys9me3) (1:500, mouse, monoclonal, FUJIFILM Cat# MABI0308).

Techniques: Immunostaining, Expressing, Staining, Activation Assay, Modification